High-Throughput Screening of Charge Switchable Lipids Discovers NonReactogenic LNPs with Extrahepatic Tropism

  • We develop a new high-throughput screening strategy that can screen LNP libraries and accurately predict delivery in vivo directly from tissue sequencing
  • Our strategy can screen LNPs in organs that traditional screening methods are impractical, such as the heart, kidneys, and brain
  • We use our strategy to screen a new class of lipids to identify LNPs with extrahepatic tropisms and low reactogenicity